Surrogacy with three parent IVF

Surrogacy, a practice dating back centuries, has evolved significantly in recent decades, particularly with the integration of advanced reproductive technologies like in vitro fertilization (IVF). More recently, the concept of “three-parent IVF” has emerged, offering novel solutions to address genetic disorders and infertility.  A genetic disease arises from abnormal genetic mutations passed down through generations, Read more about Surrogacy with three parent IVF[…]

genetic test be a solution to pursue IVF successfully in Endometriosis

Can genetic test be a solution to pursue IVF successfully in Endometriosis

Endometrial-like tissue development and progression at the exterior of the uterus often cause the chronic inflammatory condition and subsequent adhesions and scar tissue deform the pelvic anatomy of the affected women. Almost 6 to 8 percent of women globally are diagnosed with endometriosis every year. Besides other symptoms, 30 to 50 percent of women with Read more about Can genetic test be a solution to pursue IVF successfully in Endometriosis[…]

How Green Tea And Olive Oil Can Increase IVF Success Rate? (img)

How Green Tea And Olive Oil Can Increase IVF Success Rate?

Infertility issues are growing day by day which results in complications in conceiving a child. Infertility can be seen both in men and women. There are several factors that affect our fertility, but if we take the proper guidance from experts about infertility issues, we will know various things that can help us to regain Read more about How Green Tea And Olive Oil Can Increase IVF Success Rate?[…]

Egg snow

What are the drawbacks of freezing eggs or sperms for future parenting?

Freezing surplus eggs and sperm for future use is a common technique used in the assisted reproductive system.

Egg snow

But some concerns need to consider of application of frozen eggs or frozen sperm for future parenting. 

Method related drawbacks

Slow freezing and vitrification are two cryopreservation techniques commonly used for egg and sperm freezing.

Currently, human sperm are cryopreserved by applying a slow freezing process, while vitrification is applied for oocytes.

Furthermore, Open Pulled Straw and Closed Pulled Straw are two vitrification systems widely used for gametes and embryos.

However, the application of the open or close method is a debatable discussion.

Some experts believe that the open system provides a better result with an acceptable contamination risk.

But some experts stated that no difference in result for both the system and the closed system is significantly safer than the open system.

Therefore, both these methods have some conflicting results.

Liquid nitrogen is widely used as a cryogenic fluid because of its ability to maintain the ultralow temperature of −196°C that is quite low than water freezing.

At this temperature, physical and chemical reactions slow down and stop the degradation of the preserved material.

But using liquid nitrogen has some drawbacks.

Many microorganisms are able to survive in cryogenic temperatures found in liquid nitrogen and many are the factors that contribute to increasing their resistance to low temperatures.

This increases the scope of cross-contamination through the transmission of pathogens between cryopreserved cell samples during storage in liquid nitrogen.

Cross-contamination is considered a potential risk in assisted reproduction techniques.

Hepatitis B virus (HBV), herpes simplex, adenovirus, and papillomavirus are some viral pathogens that can cross-contaminate the cryopreserved gametes through liquid nitrogen.

Experts are also concerned about Zika virus transmission that may be present in frozen semen for a long period and cause teratogenicity, though no study confirms this finding.

Researchers reported that LN2 vapor is a safer alternative option to liquid nitrogen for storing cryopreserved samples.

Physiological drawbacks 

The scope of cell destruction during freezing and thawing due to intracellular ice crystals formation is one of the primary drawbacks of cryopreservation of gametes.

To solve this problem, some steps are taken care of that are as follows:

  • The total solute concentration with different cryoprotectants is increasing
  • The degree of cellular dehydration is increasing
  • The speed of cooling and/or warming is changing
  • Intracellular ice crystal formation can also be solved by shifting water out of a cell.

But osmotic shock develops due to the application of cryoprotectants forces the movement of the water by osmosis and leads to dehydration of the cell that causes a detrimental change in cell volume. 

Fluctuation of physical and chemical factors during the egg or sperm freezing process has a potential impact on the DNA structure of the cell.

For instance, alteration of osmotic pressure during the freezing process causes chromosome aberrations or elevations in sister chromatid exchange frequencies in hamster ovary cells and lymphocytes.

Thus, hyperosmotic pressure may cause DNA damage or hinder DNA repair leading to chromosomal aberrations.

Freezing impacts on eggs quality 

Progressive loss of bipolar spindle structure coexisting with chromosome displacement is resultant of leaving oocytes for longer than optimum time.

Egg freezing and/or pre-freezing influences alteration either in DNA structure or egg components implicated in repair mechanisms.

Many research studies found that cryopreservation leads to DNA fragmentation.

However, the same method does not apply for egg quality assessment after cryopreservation. Therefore, it is difficult to state that a clear conclusion.  

Freezing impacts on sperm quality 

Some research findings reported that the freezing technique modifies the DNA integrity of human sperm.

Glycerol is a primary cryoprotectant used for commercial purposes.


Three parent Eggs creation for surrogacy process

An abnormal genetic mutation associated inherited disease condition is termed a genetic disease. Genetic disorders are several different types based on the risk associated with the inheriting transmission of the disease through the next generation and complications of pregnancy. Mitochondrial disease occurs due to mtDNA mutation or nuclear DNA becoming mutated which is involved in Read more about Three parent Eggs creation for surrogacy process[…]

Surrogate Vs test-tube baby

Difference between Surrogacy and Test Tube Baby

Every woman has a common dream to enjoy motherhood. But poor lifestyle factors impact a lot on the human reproductive system. Therefore, couples are often facing infertility issues. Women experience difficulty conceiving naturally after one year of unprotected regular intercourse. Clinically this health problem is termed infertility. Approximately 50 million couples across the world are Read more about Difference between Surrogacy and Test Tube Baby[…]

Infertility treatments and alternative options in 2019 Image | Green leaf and medicine

Infertility treatments and alternative options in 2021

Thousands of couples seek infertility treatment to resolve conceiving issues or achieve childbearing capacity. The conventional treatment options have complex procedures and country-specific infertility treatment guidelines should be referred to before initiating treatment. However, there is no specific medical recommendation related to treatment commencement, definite diagnostic test or termination of treatment, etc. The infertility treatment Read more about Infertility treatments and alternative options in 2021[…]

Frozen embryo for pregnancy : Pregnant lady in red cloths

Frozen embryo: Better option to Successful pregnancy outcome

In recent days, there are many triggering factors that require preserving women’s fertility, and assisted reproductive technology (ART) becomes a very successful medical intervention. The Discovery and development of cryopreservation of embryos in the field of ART become revolutionary. For a few decades, the rate of implantation of a frozen embryo is increasing and become Read more about Frozen embryo: Better option to Successful pregnancy outcome[…]

Georgia for Surrogacy Services, Subrogada en Georgia

Top Reasons to opt for Georgian Surrogacy clinics

Georgia(Country) is best known for affordable Surrogacy is the right choice to fulfill their desire and to make their dream come true. Intended parents and couples, single parents, and those who are planning to form a family through surrogacy. Where a surrogate mother in Georgia helps them for a noble cause and some financial benefits. Read more about Top Reasons to opt for Georgian Surrogacy clinics[…]

Who requires surrogacy

IVF Process: Only option to Get Pregnant

There has been a lot of discussion about surrogacy these days. Many people, globally, who have not had any real need to discover surrogacy, might be baffled by exactly what is done for the surrogate mom to become pregnant. It frequently occurs that strangers think that some kind of “hanky panky” goes on between the Read more about IVF Process: Only option to Get Pregnant[…]